A rapid bile solubility test for pneumococci.

A rapid bile solubility test for pneumococci.

The observation that pneumococci when grown in an anaerobic atmosphere with 10% CO2 produce characteristically large colonies (Howden, 1976) called for a simple rapid confirmatory test; in this way a precise report could be issued on the morning of isolation. Hawn and Beebe (1965) described a solubility test for aerobic pneumococcal colonies using a sodium deoxycholate solution (pH 70). Aerobic...

Rapid Method for Demonstrating Bile Solubility of Diplococcus Pneumoniae.

A time-honored method for the differentation between Diplococcus pneurnoniae and Streptococcus, Viridans group), is the solubility of the former in bile or solutions of bile salts. A solution of sodium lauryl sulfate is often used, but is apparently less specific than that of sodium deoxycholate. We wished to simplify the accepted procedures, to avoid the necessity for subculture from the origi...

Rapid solubility test for detection of hemoglobin S.

Several screening methods for detecting sickling hemoglobins are presently used in the clinical laboratory (1-6). The present procedure depends on the insolubility of reduced hemoglobin S in a 2.24 mol/liter phosphate buffer. The commercially available “Sickledex” (Ortho Diagnostics, Raritan, N.J. 08869) is also thought to be based on this principle (6). Our method was compared with the standar...

Simple, rapid latex agglutination test for serotyping of pneumococci (Pneumotest-Latex).

The "gold standard" for epidemiological typing of Streptococcus pneumoniae (pneumococcus) is the capsular reaction test (Neufeld test) with antisera against the 90 pneumococcal polysaccharide capsules, i.e., serotyping. The method is labor intensive and requires a certain level of experience to be performed satisfactory, and thus it has been restricted for use in specialized reference or resear...

False positive results with a rapid solubility test for haemoglobin S.